Figure 3.

Immunofluorescent microscopy labeling of a Müller's muscle (MM) specimen, which demonstrates both myelinated and unmyelinated fibers in the MM. (A–C) Anti-peripherin (A, green) Ig stains all axons; anti-neural cell adhesion molecule (NCAM) Ig (B, red) stains non-myelinating Schwann cells. Double labeling demonstrates the presence of unmyelinated axons (C, yellow in merged image). (D, E) Anti-peripherin (D, green) labels all axons; anti-myelin basic protein (E, red) labels myelinating Schwann cells. Double labeling denotes the presence of myelinated axons (F, arrow, yellow in merged image).