Figure 4.

Immunofluorescent microscopy demonstrates labeling of Müller muscle (MM) with anti-peripherin (green) and anti-choline-acetyltransferase (ChAT) (red) immunoglobulins. Anti-peripherin Ig labels all axons; anti-ChAT labels structures positive for ChAT, such as skeletal motor nerves. Green structures represent positive anti-perpherin immunolabeling of axons (green). Note weak ChAT staining in the muscle fibers. None of the axons in this sample, as well as all other samples, were positive for ChAT, ruling out the presence of motor axons.