Table 1.

Gene regions and respective primer pairs used in the study.

Locus	PCR primers	PCR: thermal cycles: (Annealing temperature in bold)	Reference
ITS	ITS5/ITS4	(94 °C: 30 s, 55 °C: 30 s, 72 °C: 45 s) × 29 cycles	White et al. 1990
LSU	LR0R/LR5	(94 °C: 30 s, 48 °C: 50 s, 72 °C: 1 min 30 s) × 35 cycles	Vilgalys and Hester 1990; Cubeta et al. 1991
tef1	EF1-728F/EF2	(95 °C: 30 s, 51 °C: 30 s, 72 °C: 1 min) × 35 cycles	O’Donnell et al. 1998; Carbone and Kohn 1999
tub2	Bt-2a/Bt-2b	(95 °C: 30 s, 56 °C: 30 s, 72 °C: 1 min) × 35 cycles	Glass and Donaldson 1995