FIG. 2.

Inhibition of 16E2 transcriptional activation function by Rep68 and Rep40. (A) Schematic representation of the reporter construct for CAT assays. The CAT reporter gene is under the control of the thymidine kinase (TK) promoter and is followed by the simian virus 40 polyadenylation site (SV40pA). The E2-responsive enhancer consists of the HPV16 URR from nucleotides 7403 to 114 (6). (B) CAT assay. The E2-dependent CAT reporter was cotransfected in U2OS cells with or without a 16E2-expressing vector, as indicated. Full-length Rep68 as well as Rep40 inhibited basal and E2-mediated stimulation of CAT activity. Expression of the N terminus of Rep68 or Rep78 did not show any inhibitory activity. The data represent the average of three independent experiments and are reported as means and standard deviations.