FIG. 4.

Interaction between 16E2 and Rep involves the N terminus of 16E2 and the C terminus of Rep. (A) IVT 16E2 associates with GST-Rep40 but not with GST-Rep68N or GST. (B) IVT Rep68 associates with GST-16E2N but not with 16E2C or GST. Binding reactions in panels A and B were carried out as described in the legend to Fig. 3. (C) Schematic representation of 16E2 and the constructs used in this study. The N-terminal activation domain (amino acids 1 to 140) and the C-terminal DNA binding and dimerization domain (amino acids 202 to 365) were used as GST fusion proteins and as IVT products. A 16E2 deletion mutant (amino acids 156 to 159) which has lost the ability to bind 16E1 is also indicated [16E2Δ(156–159)].