Fig. 2.
Loss of GldA function is associated with upregulated expression of adjacent genes. Glycerol dehydrogenase activity in S. pyogenes M1global is abrogated following inactivation of the gldA gene, to the level observed in M1UK. Activity in the parent strain was greatest when cultured in the presence of glycerol (a) and less in glucose (b). Data show eight or nine individual reactions for each strain. Deletion of the gldA gene resulted in markedly reduced transcription of gldA (c), and upregulated transcription of mipB (d), pflD (e) and PTS component IIC (f). Data show three biological replicates per strain. Error bars show sd of the mean. *P<0.05; **P<0.01; ****P<0.0001 using a Mann–Whitney U test (a, b) and unpaired t-test (c–f).