FIG 4.

Pathogen Inhibition assay. To analyze the effect of B. subtilis on the pathogen Clostridium perfringens, the pathogenic strain was plated onto a Caso-Yeast agar plate. Small holes were punched into the agar and similar amounts of liquid Bacillus cultures or only medium as negative control were filled into them. The plates were incubated for 24 h under anaerobic conditions. The culture diffuses into the agar and inhibits the growth of C. perfringens around the holes. In comparison to the other two tested Bacillus strains, the culture of B. subtilis DSM 32315 shows the biggest inhibition halo and therefore the highest inhibition of C. perfringens.