FIG 5.

Knockdown of ct441/tsp does not alter replication or production of an elementary body (EB)-specific marker. (A) Quantification of gDNA determined by qPCR following ct441/tsp KD or NT control conditions. Samples were induced at 4 hpi with 20 nM aTc, and gDNA was harvested at 24 or 48 hpi. Samples were normalized to the genome copy number at 24 hpi and presented as fold change from 24 to 48 hpi. Dotted line represents the normalization of the 24-hpi sample to 1. ns, not significant by paired Student’s t test. (B) Western blot of HctB (EBs only) and MOMP (all Ctr) levels from uninduced and induced cultures of KD or NT knockdown strains. Samples were induced at 4 hpi with 20 nM aTc, and protein was harvested at 48 hpi. Blot is representative of three biological replicates. (C) HctB levels quantified from Western blots shown in panel B. Levels are displayed as the HctB to MOMP ratio of induced samples relative to uninduced samples. Individual points represent biological replicates. ns, not significant by paired Student’s t test.