Table 2. Cases of discrepancies between morphological and molecular-based identifications.
| Discrepancy | Morphotype | Nr. of sequences | Morphological ID of specimen | BIN | Molecular ID linked to BIN |
|---|---|---|---|---|---|
| Type 1 | “Acricotopus lucens” | 2 | Acricotopus lucens | BOLD:AAG5487 | Procladius crassinervis |
| “Chironomus” | 1 | Chironomus plumosus | BOLD:ACT6966 | Chironomus obtusidens | |
| “Chironomus” | 1 | Chironomus prasinatus | BOLD:AAU4046 | Chironomus annularius | |
| “Chironomus” | 1 | Chironomus sp. | BOLD:ADF1214 | Benthalia carbonaria | |
| “Dicrotendipes” | 1 | Dicrotendipes tritomus | BOLD:AAU1021 | Dicrotendipes nervosus | |
| “Endochironomus” | 2 | Endochironomus albipennis | BOLD:AAW5643 | Endochironomus tendens | |
| “Endochironomus” | 1 | Endochironomus stackelbergi | BOLD:AAW5643 | Endochironomus tendens | |
| “Glyptotendipes” | 1 | Glyptotendipes cauliginellus | BOLD:ACD4470 | Glyptotendipes pallens | |
| “Glyptotendipes” | 1 | Glyptotendipes glaucus | BOLD:ACD4470 | Glyptotendipes pallens | |
| “Glyptotendipes” | 1 | Glyptotendipes glaucus | BOLD:AAC0597 | Glyptotendipes paripes | |
| “Parachironomus” | 3 | Parachironomus gracilior | BOLD:ACY5073 | Parachironomus monochromus | |
| “Paratanytarsus/Rheotanytarsus” | 1 | Paratanytarsus laetipes | BOLD:AAI6018 | Cricotopus bicinctus | |
| “Procladius ferrugineus” | 2 | Procladius ferrugineus | BOLD:AAG5487 | Procladius crassinervis | |
| “Procladius pectinatus” | 1 | Procladius pectinatus | BOLD:ACW5385 | Procladius culiciformis | |
| “Pseudosmittia obtusa” | 1 | Pseudosmittia obtusa | BOLD:ACP4407 | Pseudosmittia trilobata | |
| “Smittia aterrima” | 2 | Smittia aterrima | BOLD:AAN5358 | Smittia stercoraria | |
| “Tanypus punctipennis” | 1 | Tanypus punctipennis | BOLD:ADJ7832 | Tanypus kraatzi | |
| “Tanytarsus” | 1 | Tanytarsus reei | BOLD:ACF7553 | Tanytarsus heusdensis | |
| “Tanytarsus” | 2 | Tanytarsus dispar | BOLD:ACG9929 | Tanytarsus medius | |
| “Xenopelopia nigricans” | 1 | Xenopelopia nigricans | BOLD:ADJ7832 | Tanypus kraatzi | |
| Type 2 | “Ablabesmyia phatta” | 1 | Ablabesmyia phatta | BOLD:ACK3818 | Ablabesmyia sp. 2ES |
| “Chironomidae” | 12 | Chironomidae sp. | BOLD:AAC0597 | Glyptotendipes paripes | |
| “Cladopelma/Cryptochironomus/Harnischia” | 1 | Cladopelma sp. | BOLD:AAV3586 | Cladopelma viridulum | |
| “Cladopelma/Cryptochironomus/Harnischia” | 1 | Cladopelma sp. | BOLD:AAV8096 | Cladopelma virescens | |
| “Endochironomus” | 9 | Endochironomus sp. | BOLD:AAW5643 | Endochironomus tendens | |
| “Glyptotendipes” | 1 | Glyptotendipes sp. | BOLD:ACD4470 | Glyptotendipes pallenses | |
| “Psectrocladius” | 1 | Psectrocladius sp. | BOLD:AAU0273 | Psectrocladius limbatellus | |
| “Smittia terrestris” | 2 | Smittia terrestris | BOLD:ACP4736 | Interim species Smittia sp. 8ES | |
| “Smittia terrestris” | 7 | Smittia terrestris | BOLD:ACW5117 | Interim species Smittia sp. 14ES | |
| “Thienemanniella” | 1 | Thienemanniella vittata | BOLD:AAV3048 | Interim species Thienemanniella sp. 3TE | |
| Type 3 | “Acricotopus lucens” | 1 | Acricotopus lucens | BOLD:AEO5089 | No public data |
| “Chironomus” | 6 | Chironomus curabilis | BOLD:ACD8415 | Chironomus curabilis/nuditarsis | |
| “Cricotopus” | 2 | Cricotopus sp. | BOLD:AEO5089 | No public data | |
| “Cricotopus” | 3 | Cricotopus sylvestris | BOLD:AAA5299 | Cricotopus sylvestris/glacialis | |
| “Cricotopus” | 1 | Cricotopus tricinctus | BOLD:AEG4456 | Cricotopus tricinctus/sylvestris/trifasciatus | |
| “Cricotopus” | 9 | Cricotopus sylvestris | BOLD:AAA5299 | Cricotopus sylvestris/glacialis | |
| “Glyptotendipes” | 4 | Glyptotendipes cauliginellus | BOLD:AAF8348 | Glyptotendipes cauliginellus/lobiferus | |
| “Metriocnemus” | 1 | Metriocnemus sp. | BOLD:ADV3586 | No public data | |
| “Microtendipes chloris” | 5 | Microtendipes chloris | BOLD:ACY5270 | Microtendipes pedellus/chloris | |
| “Parachironomus” | 1 | Parachironomus sp. | BOLD:ADV3586 | No public data | |
| “Procladius crassinervis” | 4 | Procladius crassinervis | BOLD:ACB6320 | Procladius sp. | |
| “Psectrocladius oxyura” | 1 | Psectrocladius oxyura | BOLD:AEO4348 | No public data | |
| “Tanytarsus usmaensis” | 2 | Tanytarsus usmaensis | BOLD:AEO0788 | No public data |
Note:
Morphotypes, number of sequences, and identifications that were involved in discrepant results, namely complete incongruences in identification across methods (type 1), molecular methods provided more species-level information than morphology (type 2), and Morphology provided more species-level information while molecular methods provided inconclusive or no identification at all (type 3).