FIG. 1.

Transcriptional analysis. (A) Schematic diagrams of the G4L and adjacent ORFs with arrows indicating direction of transcription. The size and position of the uniformly ³²P-labeled complementary G4L riboprobe relative to the ORFs are shown. nt, nucleotide. (B) RNase protection assays of G4L and F17R transcripts. Total RNA, extracted from 0 to 24 h after vaccinia virus infection of BS-C-1 cells, was hybridized with the ³²P-labeled riboprobes specific for G4L or F17R sequences. Following RNase digestion, the protected probe fragments were analyzed by PAGE and autoradiography. The marker track (M) is a 50-nucleotide end-labeled DNA ladder (sizes in nucleotides on the left). The predicted protected fragment for each gene is indicated on the right.