FIG. 5.

Detection of G4L by immunofluorescence. HeLa cells were uninfected (Q to T) or infected with vG4Li in the presence of inducer for 10 h (A to D) or 16 h (I to L) or in the absence of inducer for 10 h (E to H) or 16 h (M to P). After 10 or 16 h, the cells were fixed; permeabilized; stained with G4L peptide antiserum and an antirabbit rhodamine conjugate (B, F, J, N, and R), an anti-PDI MAb and an anti-mouse Oregon green conjugate (A, E, I, M, and Q), or Hoechst stain (blue) (C, G, K, O, and S); and viewed by confocal microscopy. Merged images of cells stained with anti-PDI, anti-G4L, and Hoechst stain are also shown (D, H, L, P, and T).