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trans complementation of vG4Li. BS-C-1 cells were infected with vG4Li at a multiplicity of 5 in the presence (+) or absence (−) of 50 μM IPTG and then mock transfected (M), transfected with pUC-19 (P), or transfected with plasmids containing the G4L gene under its natural promoter (N) or a synthetic early-late viral promoter (E/L). After 24 h, the cells were harvested and the virus titers were determined by plaque assay in the presence of 50 μM IPTG.
