FIG. 9.

Synthesis and processing of viral late proteins. BS-C-1 cells were infected at a multiplicity of 5 in the presence (+) or absence (−) of 50 μM IPTG. At 8.75 h after infection, the cells were labeled with [³⁵S]methionine for 1 h. One set of cells (pulse) were harvested, and another set were incubated with excess unlabeled methionine for an additional 14 h (chase). The proteins were analyzed by SDS-PAGE and autoradiography. Arrows indicate bands that increase during the chase in the presence of IPTG. The positions of the major core precursor proteins (p4a and p4b) and their mature processed forms (4a and 4b) are indicated on the right.