FIG. 4.
Inoculation of the recombinant YF-pOva-8 eliminates established B16 solid tumors. C57BL/6 mice were injected s.c. with melanoma B16-Ova cells (5 × 103 or 5 × 104 cells/mouse) at day 0 (tumor implantation). Animals received three s.c. inoculations every 3 days of either PBS (naïve), the parental virus 17D (YF-17D), or YF-pOva-8 (4 × 105 PFU/mouse). Viruses were administered at the day of tumor cell implantation (day 0) or 5 (day 5) or 10 (day 10) days after tumor cell inoculation. Mice were monitored for evidence of tumor growth by palpation and inspection twice a week.