Fig 3. Decoupling of curli gene expression from c-di-GMP regulation in the absence of PdeR/ DgcM regulatory module.

E. coli wild-type (WT) cells or cells lacking c-di-GMP regulatory enzymes (ΔpdeH ΔdgcE ΔpdeR ΔdgcM) were transformed with either empty pTrc99a plasmid (control) or with pTrc99a plasmid carrying dgcE (pTrc99a::dgcE) or pdeH (pTrc99a::pdeH) genes. Gene expression was induced with 1 μM IPTG. Bacteria were grown in TB overnight in flasks with shaking and cultures were subjected to the flow cytometry analysis. Fraction of positive cells in the population (mean of three biological replicates ± SEM) is indicated for each strain.