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. 2023 May 25;19(5):e1011370. doi: 10.1371/journal.ppat.1011370

Fig 1. PAM1.4-binding profile to genetically diverse VAR2CSA recombinants.

Fig 1

VAR2CSA protein sequence diversity of the 7 full-length VAR2CSA ectodomain has been analysed to highlight (A) sequence identity within the 7 phylogenetically distant alleles of VAR2CSA, reported in Renn and colleagues [12], by multiple alignment of NTS-DBL6 fragments (ranged from 2,640 to 2,723 amino acids) using BioEdit version 7.0.5.3 software. (B) The structure of VAR2CSA complex with PAM1.4 (PDB:7Z12) reported in Raghavan and colleagues [11] was used to map the PAMVAC (in blue) and PRIMVAC (in red) vaccine boundaries, while VH:VL chains of PAM1:4 are highlighted in orange and green. (C) The binding level of human monoclonal IgG PAM1.4 and (D) pooled purified IgG from MG to the 7 full-length VAR2CSA (Fv2) recombinants was measured by ELISA. OD units are reported. IgG, immunoglobulin G; MG, multigravidae; OD, optical density.