Figure 4. Contribution of site IQ to overall superoxide/hydrogen peroxide production on glycerol-3-phosphate during reverse electron transport (site IQr) and forward electron transport (site IQf).
Superoxide/hydrogen peroxide production by rat skeletal muscle mitochondria oxidising glycerol-3-phosphate alone (reverse electron transport (RET), site IQr) or in the presence of 50 µM glutamate plus malate (forward electron transport (FET), site IQf), measured in plate-reader assays in the presence of 2 µM atpenin A5 to prevent electron backflow into site IIF and 10 µM S3QEL3 to suppress site IIIQo. Grey bars show overall measured rates of hydrogen peroxide production; orange and blue bars stacked within the grey bars show the rates suppressed by the addition of 1 µM S1QEL2.1. Values are means ± SEM (n = 3 independent experiments each the mean of at least three technical replicates).