FIG. 7.

Localization of Crg-2/IP-10 gene transcripts by ISH in brains of BDV-infected β2m^0/0 MRL mice. Mice infected as newborns were sacrificed at 9 weeks of age, and one brain hemisphere each was fixed in Zamboni's fixative and processed for analysis by ISH with a ³³P-radiolabeled riboprobe specific for Crg-2/IP-10 transcripts. Brain sections were stained simultaneously with antisera (α) to BDV p40 (A and B) and GFAP (C). (A) Typical cluster of Crg-2/IP-10-positive cells in the neocortex at a low magnification. (B and C) Most Crg-2/IP-10-positive cells did not express detectable amounts of viral antigen (B), and high densities of silver grains were found most frequently over GFAP-positive cells (C). Magnifications, ∼×54 (A) and ×134 (B and C) (original magnifications were ×80 [A] and ×200 [B and C]).