FIG. 9.

Scattered distribution of astrocytes expressing the Crg-2/IP-10 gene in brains of BDV-infected triple knockout mice. A paraffin-embedded brain hemisphere of an infected mouse sacrificed 4 weeks p.i. was subjected to ISH with a ³³P-radiolabeled riboprobe specific for Crg-2/IP-10 transcripts and immunostaining with antiserum (α) to either BDV p40 (A) or GFAP (B). Some scattered Crg-2/IP-10-positive cells were present in the hippocampus (A and B). High concentrations of silver grains were typically found over GFAP-positive cells (arrowhead in panel B). Magnification, ∼×107 (original magnification, ×120).