TABLE 1.

Specifications of plasmids used for RPA and ISH studies

Gene	Subcloned sequence (position in gene)	Length (bp)	Vector	Polymerase and restriction enzyme used for antisense (sense) transcript	GenBank accession no.	Source or reference
Lymphotactin	20–240	221	pGEM-3Z	T7, HindIII	U23377	M. L. Jones (unpublished data)
MIP-1β	26–220a	195	pGEM-3Z	T7, HindIII	U06434	Jones, unpublished
	90–274	185		T7, HindIII
RANTES	73–238	166	pGEM-3Z	T7, HindIII	U06436	Jones, unpublished
MCP-1	134–279	146	pGEM-3Z	T7, HindIII	M57441	79
Mob-1/IP-10	243–372 (RPA)	130	pGEM-3Z	T7, HindIII	U22520	77
	89–553 (ISH)	464	pGEM-3Z	T7, HindIII (SP6, EcoRI)
Crg-2/IP-10b	34–399 (ISH)	355	pBS-KS	T7, EcoRI (T3, XbaI)	M86829	75
T3δ	75–310	236	pBS-SK(−)	T7, EcoRI	X53430	18, 69
T3δb	299–339, 12–230, and 675–727	342	pGEM-3Z	T7, HindIII	M12728 and M12729	74
BDV p40	714–869	155	pGEM-4Z	T7, EcoRI	L27077	17
pMulL-1βb	255–397	143	pGEM-4Z	T7, EcoRI	M15131	40
pMuTNFαb	450–753	304	pGEM-4Z	T7, EcoRI	M11731	40
L32b	1219–1293 and 1931–1954	99	pGEM-3Z	T7, HindIII	K02060	21

^aThe plasmid representing this sequence was used for RPA analysis of chemokine expression in PTI-NB rat brains. For all other RPAs, a plasmid representing the region from positions 90 to 274 was used. 

^bMouse genes. T3δ, gene encoding the delta chain of the T-cell receptor–CD3 complex. L32, gene encoding ribosomal protein L32.