FIGURE 6.

Rps14 promotes hair cell reprogramming in postnatal cochleae. (A) Experimental design. (B) Representative Myosin7a immunostaining (magenta) in the apical, middle and basal turns of cochleae transduced by AAV‐mNeonGreen and AAV‐Rps14 at the same dose (9 × 10¹⁰ GCs per cochlea). Myosin7a (magenta) marks hair cells. Cochleae were harvested at P9 and P16 after microinjection with 1.5 μL of AAV stock solution in the left ear at P2. Scale bars, 50 μm. Yellow triangles indicate the ectopic hair cells. (C,D) The number of ectopic hair cells in P9 (C) (n = 4) and P16 (D) (n = 3) cochleae corresponding to (B). The results are shown as the mean ± SEM. The p‐value was calculated by Student's t‐test (*p < 0.05; **p < 0.01; n.s. refers to no significance.). AAV, adeno‐associated virus