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. 2023 May 25;14:3007. doi: 10.1038/s41467-023-38771-4

Fig. 1. HDAC9 was significantly increased in mouse fibrotic kidneys, especially in proximal tubules.

Fig. 1

a Volcano plot showing HDACs expression in the cortex of kidney from AAN (n = 4 mice for sham group, n = 6 mice for AAN group). Statistically significant changes are marked to indicate whether genes are expected to increase (red) or decrease (blue). b Relative mRNA level of HDAC9 in the cortex of kidney from AAN (n = 10 mice per group) and UUO (n = 6 mice per group) mice. c Protein levels of HDAC9 in the cortex of kidney from AAN and UUO mice (n = 6 mice per group). d Photomicrographs and quantifications showing HDAC9 expression in kidney from AAN (n = 12 mice per group) and UUO (n = 6 mice per group) mice. Scale bar: black = 50 μm. Correlation between HDAC9 expression and the degree of Vimentin (e) or α-SMA (f) staining in AAN mice. (n = 12 mice). HDAC9 expression in proximal tubules from AAN mice (n = 6 mice per group). Aquaporin 1 (AQP1) (g) and lotus tetragonolobus lectin (LTL) (h) were used as a proximal tubular marker. Scale bar: white = 20 μm, yellow = 10 μm. i HDAC9 expression in tubules from AAN mice (n = 6 mice per group). Tamm-Horsfall glycoprotein (THP) was used as a marker for ascending loop of Henle; calbindin D28k was used as a marker for distal convoluted tubule; dolichos biflorus agglutinin (DBA) was used as a marker for collecting duct. Scale bar: white = 20 μm, yellow = 10 μm. j Protein levels of HDAC9 in human tubule epithelial cells (HK-2) with aristolochic acid (AA) treatment for 48 h. (n = 6 biologically independent experiments). Data are expressed as mean ± SEM (b, c, d, g, h, i and j). Two-tailed Student’s unpaired t test analysis (b, c, d, g, h and i), one-way ANOVA followed by Tukey’s post-test (j), nonparametric Spearman’s correlation coefficient r with two-tailed p-value (e and f). Source data are provided as a Source Data file.