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. 2023 May 25;13:8436. doi: 10.1038/s41598-023-35547-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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AAV9 (eGFP; Green) and AAV9-NEM (mScarlet; Red) transduce separate populations in murine liver. (A) eGFP and mScarlet positive area of mouse livers. Double positive areas were minimal at 4- and 8-week timepoints. (B) Representative 10× images of (A) quantification. eGFP = AAV9, mScarlet = AAV9-NEM. (C) Immunofluorescent staining for Endomucin (Emcn), F4/80, or Albumin (Purple) along with AAV9 (eGFP) and AAV9-NEM (mScarlet) cells in serial liver sections. White arrows indicate sinusoidal, Emcn-positive vascular structures, and gray arrows identify some puncta (Kupffer Cells) that are F4/80 positive. Scale bars = 50 µm. (D) Quantification of AAV9 (eGFP) or AAV9-NEM (mScarlet) positive liver tissue that is Emcn, F4/80, or Albumin positive. Comparisons via One-way ANOVA, but Week 8 AAV9-eGFP data compared by Kruskal–Wallace test. AAV dose for the mice is 1.89 × 10¹¹ vg/kg.