Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 May 25;13:8436. doi: 10.1038/s41598-023-35547-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

AAV9 and AAV9-NEM transduce separate populations in murine bone marrow. (A) Flow cytometry analysis of live eGFP, mScarlet, and double positive bone marrow cells. (B) GFP and mScarlet positive area of mouse femurs. Double positive areas were minimal at 4- and 8-week timepoints. (C) Representative 10× images. Notice the localization of eGFP (marrow) and mScarlet (bone lining interface) eGFP = AAV9, mScarlet = AAV9-NEM; scale bars = 100 µm. (D) Immunofluorescent staining for Endomucin (Emcn) or F4/80 (Purple) along with AAV9 (eGFP) and AAV9-NEM (mScarlet) cells; scale bars = 50 µm. One-way ANOVA for comparisons. N = 4 for 4-week experiment, N = 3 for 8-week experiment. AAV dose for the mice is 1.89 × 10¹¹ vg/kg.