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. 2023 May 26;8:190. doi: 10.1038/s41392-023-01415-6

Fig. 5.

Fig. 5

3HB Affects the Expression of Genes Regulated by PPARγ Ser273 Phosphorylation in 3T3-L1 Adipocytes. a, b Gene expression of adipocytes with or without siRNA knockdown of HCAR2 after 6 h 5 mM 3HB or 0.5 μM rosiglitazone (Rosi) treatment. c, d Gene expression of adipocytes treated with 5 mM 3HB or 0.5 μM for 6 h in the presence of 10 μM SCH779284 or 20 μM GW9662. e Relative HCAR2 expression of adipocytes with or without siRNA knockdown of HCAR2. f 3T3-L1 adipocytes with or without siRNA knockdown of HCAR2 were treated with 3HB or 0.5 μM rosiglitazone (Rosi) for 48 h in the absence or presence of 10 μM SCH779284 or 20 μM GW9662. Adiponectin, GLUT4, HCAR2 and GAPDH were detected by corresponding antibodies. g Adiponectin and GLUT4 protein expression of db/db mice adipose tissue. h Schematic diagram of the mechanism by which 3HB ameliorates insulin resistance in type 2 diabetic mice. The results (expressed as the mean ± SD, n = 3) were presented relative to Ctrl groups for each gene, *p < 0.05, and ***p < 0.0001