REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
FITC anti-human CD3 (clone: UCHT1)	Biolegend	Cat#300406; RRID: AB_314060
BV510 anti-human CD4 (clone: RPA-T4)	Biolegend	Cat#300546; RRID: AB_2563314
APC/Cy7 anti-human CD4 (clone: RPA-T4)	Biolegend	Cat#300518; RRID: AB_314086
PerCP anti-human CD8 (clone: SK1)	Biolegend	Cat#344708; RRID: AB_1967149
PE/Cy7 anti-human CD8 (clone: SK1)	Biolegend	Cat#344712; RRID: AB_2044008
APC anti-human PD-1 (clone: EH12.2H7)	Biolegend	Cat#329908; RRID: AB_940475
BV421 anti-human Tim3 (clone: F38-2E2)	Biolegend	Cat#345008; RRID: AB_11218598
PE anti-human Lag3 (clone: 11C3C65)	Biolegend	Cat#369306; RRID: AB_2629592
BV421 anti-human CD45RA (clone: HI100)	Biolegend	Cat#304130; RRID: AB_10965547
PE anti-human FOXP3 (clone: 206D)	Biolegend	Cat#320108; RRID: AB_492986
BV510 anti-human CCR7 (clone: G043H7)	Biolegend	Cat#353232; RRID: AB_2563866
FITC anti-human CD45RO (clone: UCHL1)	Biolegend	Cat#304242; RRID: AB_2564159
APC anti-human CD95 (clone: DX2)	Biolegend	Cat#305612; RRID: AB_314550
BV421 anti-human CXCR3 (clone: G025H7)	Biolegend	Cat#353716; RRID: AB_2561448
PE anti-human CD49d (clone: 9F10)	eBioscience	Cat#12049942; RRID: AB_10717245
PE/Cy7 anti-human IFN-γ (clone: 4S.B3)	Biolegend	Cat#502527; RRID: AB_1626154
PE anti-human IL-4 (clone: 8D4-8)	eBioscience	Cat#12704941; RRID: AB_1548823
BV421 anti-human IL-17A (clone: BL168)	Biolegend	Cat#512321; RRID: AB_10899566
FITC Mouse IgG2a κ isotype Ctrl (clone: MOPC-21)	Biolegend	Cat#400210; RRID: AB_326458
BV510 Mouse IgG2b κ isotype Ctrl (clone: MOPC-21)	Biolegend	Cat#400172; RRID: AB_2714004
PerCP Mouse IgG1 κ isotype Ctrl (clone: MOPC-21)	Biolegend	Cat#400148; RRID: AB_893680
APC Mouse IgG1 κ isotype Ctrl (clone: MOPC-21)	Biolegend	Cat#400122; RRID: AB_326443
BV421 Mouse IgG1 κ isotype Ctrl (clone: MOPC-21)	Biolegend	Cat#400158; RRID: AB_11150232
PE Mouse IgG1 κ isotype Ctrl (clone: MOPC-21)	Biolegend	Cat#400114; RRID: AB_2847829
PE/Cy7 Mouse IgG1 κ isotype Ctrl (clone: MOPC-21)	Biolegend	Cat#400125; RRID: AB_2861433
PE Rat IgG1 κ isotype Ctrl (clone: MOPC-21)	Biolegend	Cat#400407; RRID: AB_326513
BV421 Mouse IgG2b κ isotype Ctrl (clone: MOPC-21)	Biolegend	Cat#400342; RRID: AB_2935627
Bacterial and virus strains
E. coli DNA polymerase I	Invitrogen	Cat#18010-025
E. coli DNA Ligase	Invitrogen	Cat#18052-019
Biological samples
Whole blood from patients with lymphedema	Hiroshima University Hospital, Hiroshima Hiramatsu Hospital	N/A
Whole blood from healthy human	Hiroshima University Hospital	N/A
Chemicals, peptides, and recombinant proteins
7-Aminoactinomycin D	Biolegend	Cat#420403
Zombie-NIR	Biolegend	Cat#423105
RNase H	Invitrogen	Cat#18021-071
T4 DNA polymerase	Invitrogen	Cat#18005-025
Critical commercial assays
True-Nuclear Transcription Factor Buffer Set	Bbiolegend	Cat#424401
Cell Activation Cocktail (with Brefeldin A)	Biolegend	Cat#423303
Cyto-Fast Fix/Perm Buffer Set	Biolegend	Cat#426803
RNeasy Plus Universal Mini Kit	Qiagen	Cat#73404
Superscript III reverse transcriptase	Invitrogen	Cat#18080-085
NotI site	Takara Bio	Cat#1166B
NotI restriction enzyme	Takara Bio	Cat#1246B
KAPA 578 HiFi DNA Polymerase	Kapa Biosystems	Cat#KK2602
Nextera XT index kit v2 setA or setD	Illumina	Cat#FC-131-2001
Qubit 3.0 Fluorometer	Thermo Fisher Scientific	Cat#Q33216
Lymphoprep	STEMCELL Technologies	Cat#07851
Deposited data
T cell receptor repertoire sequences	This study	Zenodo (https://doi.org/10.5281/zenodo.7854980)
Oligonucleotides
P10EA adaptor (GGGAATTCGG)	Invitrogen	N/A
P20EA primers (TAATACGACTCCGAATTCCC)	Invitrogen	N/A
P22EA-ST1-R primers (GTCTCGTGGGCTCGGAGATG TGTATAAGAGACAGCTAATACGACTCCGAATTCCC)	Invitrogen	N/A
BSL-18E primer containing polyT18 (AAAGCG GCCGCATGCTTTTTTTTTTTTTTTTTTVN)	Invitrogen	N/A
TCR β 1st PCR CB1(2) (GAACTGGACTTGACA GCGGAACT)	This study	N/A
TCR β 2nd PCR CB2 (AGGCAGTATCTGGAGT CATTGAG)	This study	N/A
TCR β Tag PCR CB-ST1-R (TCGTCGGCAGCGTCAGAT GTGTATAAGAGACAGGCTCAAACACAGCGACCTC)	This study	N/A
Software and algorithms
BD FACSDiva	BD Biosciences	N/A
FlowJo (v10.8.1)	FlowJo, LLC, BD Biosciences	https://www.flowjo.com/
Repertoire analysis software Repertoire Genesis	Repertoire Genesis Inc.	N/A
JMP Pro 16	JMP Statistical Discovery LLC.	https://www.jmp.com/en_us/home.html
Repertoire Genesis	Kitaura, et al.55,56	N/A
Other
FACS Canto II	BD Biosciences	N/A