REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse anti-ACSA-2-APC	Miltenyi Biotec	Cat#130-116-142; RRID: AB_2727422
Rat anti-CD11b-BV421	BD	Cat#562605; RRID: AB_11152949
Mouse anti-O4-PE	Miltenyi Biotec	Cat#130-117-357; RRID: AB_2733886
Rabbit anti-IBA1	WAKO	Cat#019–19741; RRID:AB_839504
Chemicals, peptides, and recombinant proteins
Actinomycin D	Sigma-Aldrich	Cat#A1410-5 MG
Lipofectamine 2000	Thermo Fisher Scientific	Cat#11668019
Critical commercial assays
miRVana Paris Kit	Life Technologies	Cat#AM1556
miRNeasy Micro Kit	Qiagen	Cat#217084
miRCURY LNA RT Kit	Qiagen	Cat#339340
Superscript II reverse transcriptase	Thermo Fisher Scientific	Cat#18064071
Neuron Isolation Kit, mouse	Miltenyi Biotec	Cat#130-115-390
Adult Brain Dissociation Kit, mouse and rat	Miltenyi Biotec	Cat#130-107-677
ChromiumSingleCell3 v3	10X Genomics	Version 3, CG000183
ChromiumNextGEMSingleCell3	10X Genomics	Version 3.1, CG000204
Deposited data
Single-cell RNA sequencing (FASTQ and metadata)	Gene Expression Omnibus, NCBI	GEO: GSE230333
Experimental models: Cell lines
Human induced pluripotent cell line (iPSC)	Sigma-Aldrich	Cat#IPSC0028
Experimental models: Organisms/strains
C57BL/6 (C57BL/6JRj)	The Jackson Laboratory	RRID:IMSR_JAX:000664
Thy1-YFP-16 mice (B6.Cg-Tg(Thy1-YFP)16Jrs/J)	The Jackson Laboratory	RRID:IMSR_JAX:003709
Oligonucleotides
miR-132 antagomiR (locked nucleic acid (LNA)-, 3′-cholesterol-modified oligonucleotide)	Qiagen	Cat# 500150; Design ID: 280412
Scramble LNA antagomiR control	Qiagen	Cat# 500150; Design ID: 280407
miR-132 miRIDIAN mimic; UAAC AGUCUACAGCCAUGGUCG (MIMAT0000426)	Dharmacon	Cat# C-300599-06
Control miRIDIAN mimic; UCACA ACCUCCUAGAAAGAGUAGA (MIMAT0000039)	Dharmacon	Cat# CN-001000-01
Primer mmu-miR-132-3p; UAA CAGUCUACAGCCAUGGUCG	Qiagen	Cat# 204129
Primer mmu-miR-212-3p; UAAC AGUCUCCAGUCACGGCCA	Qiagen	Cat# 206022
mRNA primers (for full sequence, see Table S6)	IDT	Custom primer sets
Software and algorithms
FIJI	Schindelin et al.166	https://fiji.sc/
Encori	Li et al.62	https://starbase.sysu.edu.cn/
Magma	De Leeuw et al.165	https://ctg.cncr.nl/software/magma
Palantir	Setty et al.103	https://github.com/dpeerlab/Palantir
David	Huang et al.64	https://david.ncifcrf.gov/
Seurat	Butler et al.163	Version 4.1.1, https://satijalab.org/seurat/
Scanpy	Wolf et al.159	Version 1.8.1 https://scanpy.readthedocs.io/en/stable/
Spectronaut	Biognosys	Spectronaut 14