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. 2023 May 25;13:8146. doi: 10.1038/s41598-023-34445-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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3D muscle tissues fabricated from Puro-bulk MYOD1-hiPSCs exhibited contractile force, indicating the functionality of the hiPSC-derived muscle tissues. (a) Schematic of the fabrication of 3D muscle tissues. On day 3 of differentiation, differentiating cells were dissociated and replated on microdevices. On day 11, muscle tissues were pulled up at the top of the pillar and processed for measurement of contractile force elicited by EPS at days 11, 13, 15 and 17. (b) Brightfield top-view images of muscle tissues derived from Puro-bulk 201B7-MYOD1-hiPSCs at days 11, 13, 15, and 17. Scale bar, 500 μm. (c) IHC analysis of fabricated muscle tissues from Puro-bulk 201B7-MYOD1-hiPSCs for Titin and α-Actinin at day 17 of differentiation. Sarcomere formation was clearly observed in Puro-bulk 201B7-MYOD1-hiPSC-derived muscle tissues. The nuclei were stained with Hoechst 33258. Scale bar, 20 µm. (d) Measurement of the contractile force of the muscle tissues derived from seven hiPSC clones (201B7, 409B2, EKN3, YFE16, YFE19, TIGE9, and TIGE22) at days 11, 13, 15, and 17 of differentiation. The maximum contractile force is recorded at day 15. The data are presented as the mean ± SEM, n = 4. (e) Comparison of the maximum contractile force of muscle tissues derived from seven hiPSC clones (201B7, 409B2, EKN3, YFE16, YFE19, TIGE9, and TIGE22) and Hu5/KD3 cells.