Fig. 1.

VHH E3 and C4 with related constructs. a Sequence alignment of E3 and C4 VHHs with numbering starting after the signal peptide, although sometimes incomplete cleavage occurs and the preceding MA of the signal peptide is left attached. The Myc tag is highlighted with gray shading, 6xHis tag highlighted in purple shading, and each CDR is boxed in cyan, teal, and blue (1, 2, 3, resp.). N- and C-terminal changes for the Fc, CSA, and bivalent constructs are also shown. b Structural representation of each variation with the tags labeled and their side-chains represented as sticks, the N- and C-terminals labeled and colored (white and black resp.), as well as the CDRs labeled and color coded (similarly as in a)