Figure 11.

Analysis of ER stress and autophagy markers in intestinal epithelial-specific Ptpn2 mouse IECs. (A) Western blot of IECs from Ptpn2^fl/fl and Ptpn2^ΔIEC mice probed for lysozyme-1, Mist1, and unfolded protein response marker Xbp-1s. (B) Densitometry of lysozyme protein levels in IECs comparing Ptpn2^fl/fl and Ptpn2^ΔIEC. (C) Densitometry of Mist1 protein levels in IECs comparing Ptpn2^fl/fl and Ptpn2^ΔIEC. (D) Western blot and densitometry of IECs from Ptpn2^fl/fl and Ptpn2^ΔIEC mice probing for (E) Xbp-1s; (F) Mmp7, a Paneth cell marker; (G) downstream ER stress inducer C/EBP-homologous protein; (H) relative ratio of eukaryotic translation initiation factor 2A isoforms p-eIF2α and total eIF2α; (I) ER stress marker Bip protein; and (J) relative ratio of autophagy activation marker LC3B-II/LC3B-I. Columns show means ± SD. Two-tailed unpaired t test.