Figure 2.

Assessment of STAT activation in isolated IEC samples from Ptpn2-deficient mice. (A) Western blot of ileal IECs probing for α-smooth muscle actin (α-SMA) to assess the presence of mesenchymal cells; CD45 to assess the presence of leukocytes; and epithelial cell adhesion molecule (EpCAM) to assess an abundance of epithelial cells. (B) Western blot of ileal IECs showing the absence of TCPTP and abundance of TCPTP substrates STAT1 and STAT3 in their total and phosphorylated isoforms. WT = 5, HET = 5, KO = 5. (C) Staining of ileal mucosa by immunohistochemistry for phosphorylated STAT3. Black arrowheads indicate positive staining for phosphorylated STAT3 (p-STAT3) in the mesenchyme, whereas red arrows indicate IECs positive for p-STAT3. WT = 3, HET = 3, KO = 3.