Skip to main content
. Author manuscript; available in PMC: 2024 Jun 5.
Published in final edited form as: Angew Chem Int Ed Engl. 2023 Apr 27;62(23):e202216784. doi: 10.1002/anie.202216784

Figure 5.

Figure 5.

Immunofluorescence staining of HEK293T cells treated with (a) 10 μM puromycin (1), (b) 10 μM thpuromycin (2), (c) 10 μM AzThpuromycin (3), and (d) 10 μM F2AzThpuromycin (4) for 2 hours. Image channels top to bottom: DRAQ5 (nuclear stain), anti-puromycin (primary) and GAMAF488 (secondary), HPO-0100 (anti-ribosome, primary) and DAH Cy3 (secondary), overlay. (e) Fixed primary rat hippocampal neuron images stained with DRAQ5 (top), compound 4 (second row), anti-puromycin (third row), and anti-ribosome (fourth row). Images are overlayed (bottom).