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. 2023 May 12;16:1175851. doi: 10.3389/fnmol.2023.1175851

Figure 5.

Figure 5

Mitochondrial DNA copy number and membrane ultrastructure is not altered in Cstb−/− cerebella. (A) The relative ratio between mtDNA copy number and protein amount in synaptosomes of wild type (white) and Cstb−/− (black) mice cerebella at P30 (n = 10–13) and P45 (n = 13). Bars represent mean, and error bars standard deviation (SD) of relative mtDNA copy number/mg synaptosomal protein. (B,C) Transmission electron microscopy images showing mitochondrial ultrastructure in wild type (B) and Cstb−/− (C) cerebellar tissue at P45. (D) Representative immunoblot detection using an antibody against OPA1 in synaptosomes of wild type and Cstb−/− mice (n = 4 + 6) at P45. The long and short OPA1 isoforms are marked L1-L3 and S1-S2, respectively. (E) The short isoforms of OPA1 (S-OPA1) in relation to total OPA1 in synaptosomes of wild type (white) and Cstb−/− (black) mice at P45 (n = 8–10). Bars represent mean, and error bars standard deviation (SD).