Figure 1.

BBD decreased diethylnitrosamine-initiated hepatocellular carcinoma formation and ameliorated liver injury in rats. The rats’ hepatocarcinogenesis model was induced by intraperitoneal injections of DEN at a dose of 70 mg/kg body weight once a week for 12 weeks. BBD was administered to rats at a dose of 0.5g/kg body weight every two days from the 9^th to the 12^th week. All rats were sacrificed on the last day of 12 weeks. (A) Schematic representation of induction of experimental HCC in rats and administration. (B) Representative upper and visceral views of rat’s liver in different groups. (C) The number of rats with liver cancer in different groups. (D) The Serum levels of ALB(U/L), ALT(U/L) and AST(U/L). (E) HE staining of liver tissues. Differences were analyzed using a two-tailed Student’s t-test (Compared to DEN group, **P <0.01; compared to Normal group, N.S., no significance).