Figure 5.

BBD inhibited primary HPCs neoplastic transformation. Primary HPCs were treated with 10 μg/mL LPS supplemented with 10% normal-serum(v/v), or 10% BBD-serum groups for 2 months, respectively, to induce them to undergo neoplastic transformation. (A) Representative images of colony formation in 6-well plates in standard medium with different treated primary HPCs for 72h. (B) Quantification of Colonies number. (C) Representative images of Different treated primary HPCs cells were suspended in serum free medium were seeded and incubated for 3-7 days. (D) The size of sphere was observed manually under microscope. (E) LPS-induced primary HPCs cells treated with different serum were subcutaneously transplanted into nude mice. (F) The volume was compared. Differences were analyzed using a two-tailed Student’s t-test (Compared to normal-serum with LPS group, *P < 0.05, **P < 0.01).