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. 2023 May 12;14:1117638. doi: 10.3389/fimmu.2023.1117638

Figure 1.

Figure 1

Norbergenin (Nbn) inhibits LPS-induced inflammation. (A), Effect of Nbn at indicated concentrations on cell viability in iBMDMs. qPCR analysis of Il1β, Tnfα, Il6, and Nos2 in iBMDMs (B–E) and BMDMs (F–I) pretreated with or without Nbn at concentrations of 50 μM for 1 h prior to stimulation with LPS (200 ng/mL) for indicated time points. NO generation (J), TNFα generation (K), and IL1β and iNOS (L) measured in iBMDMs preincubated with or without Nbn for 1 h followed by stimulation with LPS (200 ng/mL) by Griess assay, ELISA, and western blotting analysis, respectively. Data are presented as mean ± SEM of n ≥ 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 (unpaired two-tailed student’s t test).