Figure 3.

Norbergenin (Nbn) attenuates LPS-activated inflammation through inhibition of NFκB and STAT3 activation. (A), Representative immunoblots of p-NFκB, NFκB, p-IκBα, IκBα measured in iBMDMs pretreated with or without Nbn for 1 h prior to incubation with LPS (200 ng/mL) for 4 h. (B), iBMDMs were pretreated with or without PDTC for 30 min in the absence or presence of 50 μM Nbn before addition of LPS (200 ng/mL), p-NFκB, NFκB, p-IκBα and IκBα were analyzed by western blotting, IL1β and iNOS were measured by western blotting and qPCR (B–D) analysis. E, Representative immunoblots of p-STAT3 and STAT3. Data are presented as mean ± SEM of n ≥ 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 (unpaired two-tailed student’s t test).