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. 2023 May 12;14:1117638. doi: 10.3389/fimmu.2023.1117638

Figure 4.

Figure 4

Norbergenin (Nbn) reduces LPS-triggered inflammation via inhibition of MAPKs in iBMDMs. iBMDMs were pretreated with or without Nbn at indicated concentrations for 1 h followed by treatment with LPS (200 ng/mL) for 8 h. (A), Expressions of p-p38, p-JNK and p-ERK protein were determined by Western blotting, and p38, JNK and ERK were used as loading controls. iBMDMs were preincubated with or without Nbn (50 μM) for 1 h in the absence or presence of SB203580, SP600125, and U0126 followed by stimulation with LPS (200 ng/mL) for 8 h. Then the protein expression of p-p38 (B), p-JNK (D), and p-ERK (F) protein were determined by Western blotting, and p38, JNK, and ERK were used as loading controls. IL1β and iNOS protein or mRNA expression was assessed by western blotting or qPCR (B–G). Data are presented as mean ± SEM of n ≥ 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 (unpaired two-tailed student’s t test).