Fig. 1.

BT-Br acts as an efficient CAT inhibitor in DU145 cells. (a) The synthesis and structural formula of BT-Br. Reagents and conditions are as following: i) NBS, NH₄OAc, MeCN, r.t.; ii) EtOH, reflux, overnight. The yields of intermediate and final product are shown in brackets. (b) The inhibition effects of different concentrations of BT-Br on CAT activity in PBS buffer, IC₅₀ = 59.05 ± 5.32 nM. (c) The relative mRNA levels of CAT upon different concentrations of BT-Br in DU145 cells are measured by RT-qPCR. (d) Western blot and the corresponding quantization of CAT/GAPDH ratios in DU145 cells in the presence of different concentrations of BT-Br (0, 0.1, 0.5, 1.0, 1.5, 2.0 μM). The Image J software is used to quantify the grayscale of bands. Error bars represent means ± SD (n = 3); Fig. 1b–d: two-tailed unpaired t-test, *p < 0.05, **p < 0.01, ***p < 0.001, or ****p < 0.0001, ns, not significant.