Fig. 6.

BT-Br induces ER stress and subsequent autophagy in DU145 cells. (a–d) Evaluation of the effects of the autophagy inhibitor CQ or BafA1 on BT-Br-induced LIP (a, c) and relative lipid ROS (b, d). (e–j) TEM images of DU145 cells treated with vehicle (1% DMSO) or 5 μM BT-Br (incubated for 4 h and 12 h, respectively). Red rectangles represent the region enlarged, the cyan arrows indicate mitochondria, and the yellow arrows indicate autophagosomes. (k) The expression of ER stress-related proteins eIF2a, P-eIF2a and CHOP in DU145 cells treated with different concentrations of BT-Br (0, 1.0, 2.5, 5.0 μM). DU145 cells were incubated with BT-Br for 24 h. The bar graphs were the corresponding quantization of protein/β-actin ratios to the Western blot images. (l) The expression of eIF2a, P-eIF2a, CHOP (l) in DU145 cells treated with 2.5 μM BT-Br with or without 1 mM 4-PBA. Error bars represent means ± SD (n = 3). Fig. 6a–d,k,l: Two-tailed unpaired t-test. *p < 0.05, **p < 0.01, ***p < 0.001, ns, not significant. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)