FIGURE 2.

FFAR1 is a candidate molecule that regulates oligodendrocyte cell death. (A) Representative images show the fluorescence of PI (red) and immunocytochemistry for MBP (green) of primary oligodendrocytes treated with vehicle, glutamate, glutamate + OA + LA, OA + LA. White arrows indicate PI⁺ MBP⁺ cells. Scale bar: 30 μm. (B) Graph indicating the percentage of PI⁺ MBP⁺ cells in MBP⁺ cells (vehicle: n = 6, glutamate: n = 6, glutamate + OA + LA: n = 6, OA + LA: n = 5). (C) Graph showing the percentage of PI⁺ MBP⁺ cells in MBP⁺ cells of primary oligodendrocyte culture transfected with siRNA library followed by treatment with glutamate + OA + LA (n = 3 for each). (D) Plot indicating the –log₁₀ (p-value) and log₂ (fold change) of PI⁺ MBP⁺ cell percentage compared with control siRNA in each siRNA treatment (n = 3 for each group). (E) Relative expression of Ffar1 mRNA in primary oligodendrocytes after control or Ffar1 siRNA treatment (n = 3 for each group). (F) The percentage of PI⁺ MBP⁺ cells in MBP⁺ cells after Control or Ffar1 siRNA treatment (n = 3 for each group). Error bars represent mean ± SEM, **P < 0.01, *P < 0.05, assessed by one-way ANOVA followed by Tukey–Kramer test for panels (B–D), and by two-sided Student’s t-test for panels (E,F), NS, not significant.