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. 2023 May 12;17:1081190. doi: 10.3389/fncel.2023.1081190

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Copyright © 2023 Maruyama, Tanabe, Uyeda, Suzuki and Muramatsu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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OA and LA inhibit glutamate-induced oligodendrocyte apoptosis via FFAR1. (A) Representative images show the fluorescence of PI (red) and immunocytochemistry for MBP (green) of purified primary oligodendrocytes treated with vehicle, glutamate, glutamate + OA/LA, and OA/LA. Scale bar: 100 μm. (B) Graph showing the percentage of PI⁺ MBP⁺ cells in MBP⁺ cells assessed from panel (A) (Vehicle: n = 4, glutamate: n = 4, glutamate + OA/LA: n = 4, and OA/LA: n = 4). (C) Representative images showing the fluorescence of PI (red) and immunocytochemistry for MBP (green) in purified primary oligodendrocytes treated with control siRNA or Ffar1 siRNA in the presence of glutamate with or without OA/LA. Scale bar: 100 μm. (D) Graph showing the percentage of PI⁺ MBP⁺ cells in MBP⁺ cells (Control siRNA in glutamate: n = 4, Control siRNA in glutamate + OA/LA: n = 4, Ffar1 siRNA in glutamate + OA/LA: n = 4, and Ffar1 siRNA in glutamate + OA/LA: n = 4). (E) Representative images showing fluorescence of immunocytochemistry for CC3 (cyan) and MBP (green) of purified primary oligodendrocytes treated with vehicle, glutamate, glutamate + OA/LA, and OA/LA. Scale bar: 100 μm. (F) Graph showing the percentage of CC3⁺ MBP⁺ cells in MBP⁺ cells assessed from panel (E) (vehicle: n = 4, glutamate: n = 4, glutamate + OA/LA: n = 4, and OA/LA: n = 4). (G) Representative images showing the fluorescence of immunocytochemistry for MBP (green) and CC3 (cyan) of purified primary oligodendrocytes treated with control siRNA or Ffar1 siRNA in the presence of glutamate with or without OA/LA. Scale bar: 100 μm. (H) Graph showing the percentage of CC3⁺ MBP⁺ cells in MBP⁺ cells assessed from panel (G) (Control siRNA in glutamate: n = 4, Control siRNA in glutamate + OA/LA: n = 4, Ffar1 siRNA in glutamate + OA/LA: n = 4, and Ffar1 siRNA in glutamate + OA/LA: n = 4). NS, not significant, *P < 0.05, **P < 0.01, assessed by one-way ANOVA followed by Tukey–Kramer test for panels (B,F), and two-way ANOVA followed by Sidak’s multiple comparison tests for panels (D,H).