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. 2023 May 12;17:1081190. doi: 10.3389/fncel.2023.1081190

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Copyright © 2023 Maruyama, Tanabe, Uyeda, Suzuki and Muramatsu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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OA and LA prevent the OL loss in SOD1^G93A mice. (A) Representative images show the immunohistochemistry for CC1 (green), Olig2 (red), and Ffar1 (cyan) in the spinal cord section of SOD1^G93A at P100. (B) Representative images indicate immunohistochemistry for CC1 (green), Olig2 (red), and CC3 (cyan) in the spinal cord of Vehicle or OA + LA injected SOD1^G93A mice at P100. White arrows indicate CC1⁺ Olig2⁺ CC3⁺ cells. (C) Graph shows the number of CC1⁺ Olig2⁺ CC3⁺ cells per mm² (n = 4 for each group). (D) Graph shows the number of CC1⁺ Olig2⁺ cells per mm² (n = 4 for each group). (E) Graph showing the gram of grip strength (vehicle: n = 3, OA/LA: n = 4). *P < 0.05 assessed by two-sided Student’s t-test for panel (C) and two-way ANOVA followed by Sidak’s multiple comparison test for panel (E). Scale bars: 25 μm for panels (A,B).