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. 2023 May 12;17:1081190. doi: 10.3389/fncel.2023.1081190

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Copyright © 2023 Maruyama, Tanabe, Uyeda, Suzuki and Muramatsu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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OA and LA prevent the degeneration of MNs in SOD1^G93A mice. (A) Representative images show the immunohistochemistry for ChAT in the spinal cord section. (B) The graph shows the number of ChAT⁺ cells per section (vehicle: n = 5, OA + LA: n = 4). (C–F) Representative images show the immunohistochemistry for Iba1 (green, C) and GFAP (red, E) in the spinal cord section. Graphs show the relative fluorescence intensity of Iba1 (D) and GFAP (F), respectively (n = 4 for each group). Error bars represent mean ± SEM, *P < 0.05 assessed by two-sided Student’s t-test. NS, not significant. Scale bars: 100 μm (A,C,E).