Table 2.
Functional enrichment and annotation analysis.
| Functional annotation | Enriched functions | P-value | Observed | miRNAs/precursors |
|---|---|---|---|---|
| Diseases (MNDR) | type 2 diabetes mellitus | 0.001218 | 4 | hsa-miR-103b; hsa-miR-191-5p; hsa-miR-24-3p; hsa-miR-200c-3p |
| Diseases (MNDR) | Diabetic Nephropathies | 0.00817 | 3 | hsa-miR-24-3p; hsa-miR-26a-5p; hsa-miR-200c-3p |
| Diseases (MNDR) | diabetes mellitus | 0.011561 | 8 | hsa-miR-151a-5p; hsa-miR-22-3p; hsa-miR-24-3p; hsa-miR-26a-5p; hsa-miR-30d-5p; hsa-miR-361-5p; hsa-miR-200c-3p; hsa-miR-574-3p |
| Expressed in tissue (Tissue Atlas) | Kidney | 0.014937 | 11 | hsa-miR-151a-5p; hsa-miR-1972; hsa-miR-22-3p; hsa-miR-24-3p; hsa-miR-26a-5p; hsa-miR-30d-5p; hsa-miR-361-5p; hsa-miR-378a-3p; hsa-miR-200c-3p; hsa-let-7i-5p; hsa-miR-574-3p |
| GO Biological process (miRPathDB) | fibroblast proliferation | 2.25e-5 | 5 | hsa-miR-22-3p; hsa-miR-24-3p; hsa-miR-26a-5p; hsa-miR-200c-3p; hsa-miR-574-3p |
| GO Biological process (miRPathDB) | positive regulation of fibroblast proliferation | 1.18e-5 | 5 | hsa-miR-22-3p; hsa-miR-24-3p; hsa-miR-26a-5p; hsa-miR-200c-3p; hsa-miR-574-3p |
| GO Biological process (miRPathDB) | regulation of fibroblast proliferation | 2.25e-5 | 5 | hsa-miR-22-3p; hsa-miR-24-3p; hsa-miR-26a-5p; hsa-miR-200c-3p; hsa-miR-574-3p |
| GO Biological process (miRPathDB) | regulation of transforming growth factor beta receptor signaling pathway | 1.82e-4 | 4 | hsa-miR-22-3p; hsa-miR-26a-5p; hsa-miR-200c-3p; hsa-miR-574-3p |
| GO Biological process (miRPathDB) | positive regulation of gluconeogenesis | 5.38e-4 | 2 | hsa-miR-22-3p; hsa-miR-200c-3p |
| GO Biological process (miRPathDB) | positive regulation of protein kinase activity | 5.07e-4 | 5 | hsa-miR-22-3p; hsa-miR-24-3p; hsa-miR-26a-5p; hsa-miR-378a-3p; hsa-miR-200c-3p |
| GO Biological process (miRPathDB) | positive regulation of cell migration by vascular endothelial growth factor signaling pathway | 8.92e-4 | 2 | hsa-miR-378a-3p; hsa-miR-200c-3p |
| GO Biological process (miRPathDB) | renal tubule morphogenesis | 0.001014 | 3 | hsa-miR-378a-3p; hsa-miR-200c-3p; hsa-miR-574-3p |
| GO Biological process (miRPathDB) | nitrogen compound metabolic process | 0.001061 | 7 | hsa-miR-22-3p; hsa-miR-24-3p; hsa-miR-26a-5p; hsa-miR-30d-5p; hsa-miR-361-5p; hsa-miR-378a-3p; hsa-miR-200c-3p |
| GO Biological process (miRPathDB) | nephron morphogenesis | 0.00116 | 3 | hsa-miR-378a-3p; hsa-miR-200c-3p; hsa-miR-574-3p |
| GO Biological process (miRPathDB) | response to insulin | 0.001319 | 3 | hsa-miR-22-3p; hsa-miR-26a-5p; hsa-miR-200c-3p |
| GO Biological process (miRPathDB) | signal transduction involved in DNA damage checkpoint | 0.001269 | 4 | hsa-miR-24-3p; hsa-miR-26a-5p; hsa-miR-200c-3p; hsa-miR-574-3p |
| GO Biological process (miRPathDB) | extrinsic apoptotic signaling pathway | 0.001561 | 4 | hsa-miR-22-3p; hsa-miR-24-3p; hsa-miR-26a-5p; hsa-miR-200c-3p |
| GO Biological process (miRPathDB) | response to growth factor | 0.002298 | 5 | hsa-miR-22-3p; hsa-miR-26a-5p; hsa-miR-378a-3p; hsa-miR-200c-3p; hsa-miR-574-3p |
| GO Biological process (miRPathDB) | Gluconeogenesis | 0.003142 | 2 | hsa-miR-22-3p; hsa-miR-200c-3p |
| GO Biological process (miRPathDB) | regulation of apoptotic process | 0.003842 | 5 | hsa-miR-22-3p; hsa-miR-24-3p; hsa-miR-26a-5p; hsa-miR-378a-3p; hsa-miR-200c-3p |
| GO Biological process (miRPathDB) | negative regulation of epithelial cell apoptotic process | 0.004749 | 2 | hsa-miR-24-3p; hsa-miR-200c-3p |
| GO Biological process (miRPathDB) | negative regulation of endothelial cell apoptotic process | 0.005669 | 2 | hsa-miR-24-3p; hsa-miR-200c-3p |
| GO Biological process (miRPathDB) | transforming growth factor beta receptor signaling pathway | 0.006793 | 3 | hsa-miR-26a-5p; hsa-miR-200c-3p; hsa-miR-574-3p |
| GO Biological process (miRPathDB) | negative regulation of cellular response to insulin stimulus | 0.01003 | 1 | hsa-miR-200c-3p |
| GO Biological process (miRPathDB) | negative regulation of gluconeogenesis | 0.01003 | 1 | hsa-miR-200c-3p |
| GO Biological process (miRPathDB) | positive regulation of vitamin D biosynthetic process | 0.01003 | 1 | hsa-miR-24-3p |
| GO Biological process (miRPathDB) | activation of protein kinase activity | 0.01239 | 3 | hsa-miR-24-3p; hsa-miR-378a-3p; hsa-miR-200c-3p |
| GO Biological process (miRPathDB) | regulation of kinase activity | 0.013831 | 4 | hsa-miR-22-3p; hsa-miR-24-3p; hsa-miR-378a-3p; hsa-miR-200c-3p |
| Pathway (miRPathDB) | Wnt signaling pathway | 4.36e-4 | 5 | hsa-miR-151a-5p; hsa-miR-22-3p; hsa-miR-26a-5p; hsa-miR-200c-3p; hsa-miR-574-3p |
| Pathway (miRPathDB) | TGF-beta signaling pathway | 0.005433 | 4 | hsa-miR-22-3p; hsa-miR-378a-3p; hsa-miR-200c-3p; hsa-miR-574-3p |
| Pathways (KEGG) | Type I diabetes mellitus | 0.001892 | 8 | hsa-miR-103b; hsa-miR-191-5p; hsa-miR-1972; hsa-miR-24-3p; hsa-miR-26a-5p; hsa-miR-30d-5p; hsa-miR-619-5p; hsa-let-7i-5p |
| Pathway (miRWalk) | Apoptosis signaling pathway | 8.32e-4 | 9 | hsa-miR-151a-5p; hsa-miR-191-5p; hsa-miR-22-3p; hsa-miR-24-3p; hsa-miR-26a-5p; hsa-miR-30d-5p; hsa-miR-361-5p; hsa-miR-378a-3p; hsa-miR-200c-3p |
Table detailed the results from the analysis including, pathways, gene ontology, expression sites and disease annotations of the miRs using miEAA 2.0 miRNA Enrichment Analysis and Annotation tool. P-value is calculated as adjusted p-value or FDR by using Benjamini–Hochberg method.