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. 2023 May 12;14:1172681. doi: 10.3389/fimmu.2023.1172681
Differentiation
Antigen Fluorochrome Company Catalog No.
CD45RA Allophycocyanin-Cy7 (APC-Cy7) Miltenyi Biotec 130-113-363
CD62L R-Phycoerythrin (PE) Miltenyi Biotec 130-113-625
CD4 Phycoerythrin-Vio770 (PE-Vio770) Miltenyi Biotec 130-113-227
CD8 Brilliant Violet 510 BD Bioscience 563919
CD95 Peridinin Chlorophyll Protein-Cy5.5 (PerCP-Cy5.5) Biolegend 305630
Viability Fixable Viability Dye eFluor 780 Invitrogen 65086514
UCAR T Phenotype
FAP Protein Fluorescein (FITC) AcroBiosystems FAP-HF263
Mesothelin protein R-Phycoerythrin (PE) AcroBiosystems MSN-HP2H8
TCRa/b Allophycocyanin (APC) Miltenyi Biotec 130-098-859
HLA-ABC VioBlue Miltenyi Biotec 130-101-459
Tumor analysis
Antigen Fluorochrome Company
hCD45 R-Phycoerythrin (PE) Miltenyi 130-110-632
mCD45 Brilliant Violet-421 (BV421) BD Biosciences 560501
CD4 Phycoerythrin-Vio770 (PE-Vio770) Miltenyi Biotec 130-113-227
CD8 Brilliant Violet 510 (BV510) BD Bioscience 563919
hFAP Alexa Fluor 488 R&D Systems MAB9727-100
hMesothelin Allophycocyanin (APC) R&D Systems FAB32652P