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MPXV genomic DNA was detected in concentrated wastewater collected weekly at the Miami-Dade Central District Wastewater Treatment Plant. MPXV target was first detected by quantitative PCR in mid-July of 2022 and remained detectable until testing negative in September. All weekly samples analyzed from June 8 through July 6, plus September 7 to September 21 were below detection limits of 180 gc/L.
