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High doses of metformin may selectively impact nutrient transporter expression. (A) SNAT1, (B) SNAT2, (C) LAT1, (D) LAT2, and (E) FATP6 gene expression by qPCR in BeWo cells treated with DMSO or Forskolin and 200 µM or 2000 µM metformin compared to vehicle. n = 4 biological replicates. Data are representative of mean ± SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.001; and ****, p < 0.0001.
