Skip to main content
. 2023 May 25;6(8):e202201853. doi: 10.26508/lsa.202201853

Figure 4. Deconvolution analysis predicts increase in aberrant basaloid like-cells by the fibrosis cocktail.

Figure 4.

(A) Schematic depicting Bisque reference-based analysis of epithelial cell populations from non-fibrotic controls and IPF from the publicly available single-cell RNA sequencing dataset (GEO Accession: GSE135893). (B) Selected epithelial cell proportions estimated by Bisque in alveolospheres stimulated with CC or FC. n = 3 batches of alveolospheres. (C) Expression of selected epithelial genes in alveolospheres. Data presented as means ± SD. **** = P(adj) < 0.0001, ** = P(adj) < 0.01. n = 3 batches of alveolospheres. (D) Immunofluorescence of alveolospheres stimulated with CC or FC. Scale bar = 50 μm. (E) Quantification of the fluorescent area of KRT17 and KRT5 over Hoechst in CC- and FC-stimulated alveolospheres. Boxplots indicate medians with minimum and maximum. n = 3–4 independent regions for each condition and each batch of alveolospheres, in total 3 batches. **** = P < 0.0001 by unpaired, two-tailed t-test. (F) Proportion of organoids with “normal” and “dense” morphologies expressing KRT17 in high levels (Hi), low levels (Lo) or no expression (Neg) upon FC stimulation. n = 8 independent images for each batch of alveolospheres, in a total of three batches. See also Fig S8.