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(A) Northern blot of pituitary GH3 and osteosarcoma UMR 106 cell poly(A)+ mRNA (5 μg, duplicate lanes) hybridized to a TRβ1 cRNA. A 6.2-kb mRNA is present in both cell types, and a 7.0-kb mRNA is present only in UMR 106 cells. (B) Southern blot of normal liver (N) and ROS 17/2.8, ROS 25/1, and UMR 106 osteosarcoma cell (lanes 17, 25, and U) genomic DNA, digested with EcoRI, HindIII, PstI, and BamHI and probed with TRβ1 (top) and exon A (bottom) probes.
